Product overview:

Carrier-free (BSA/glycerol-free) KPNB1 mouse monoclonal antibody,clone LBI10D3

Field of research:

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Summary:

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

Alternative names:

IMB1; Impnb; IPO1; IPOB; NTF97

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

KPNB1

Isotype:

IgG2a

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-200 of human KPNB1 (NP_002256) produced in E.coli.

Clone:

LBI10D3

Conjugation:

Unconjugated

Gene name:

Homo sapiens karyopherin subunit beta 1 (KPNB1), transcript variant 1, mRNA.

Symbol:

KPNB1

Pathway:

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Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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