Product overview:

Carrier-free (BSA/glycerol-free) POLR2A mouse monoclonal antibody,clone LBI1E5

Field of research:

Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

Summary:

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

Alternative names:

hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

POLR2A

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1351-1614 of human POLR2A(NP_000928) produced in E.coli.

Clone:

LBI1E5

Conjugation:

Unconjugated

Gene name:

Homo sapiens RNA polymerase II subunit A (POLR2A), mRNA.

Symbol:

POLR2A

Pathway:

Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:500

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com