Product overview:

Carrier-free (BSA/glycerol-free) MED27 mouse monoclonal antibody,clone LBI2A4

Field of research:

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Summary:

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Alternative names:

CRAP34; CRSP8; CRSP34; MED3; NEDSCAC; TRAP37

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

MED27

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 24-247 of human MED27(NP_004260) produced in E.coli.

Clone:

LBI2A4

Conjugation:

Unconjugated

Gene name:

Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.

Symbol:

MED27

Pathway:

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Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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