Product overview:

Carrier-free (BSA/glycerol-free) TRMT5 mouse monoclonal antibody,clone LBI1B5

Field of research:

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Summary:

tRNAs contain as many as 13 or 14 nucleotides that are modified posttranscriptionally by enzymes that are highly specific for particular nucleotides in the tRNA structure. TRMT5 methylates the N1 position of guanosine-37 (G37) in selected tRNAs using S-adenosyl methionine (Brule et al., 2004 [PubMed 15248782]). [supplied by OMIM, Mar 2008]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC136606.1, AK293158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns ERS025082, ERS025083 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

Alternative names:

COXPD26; KIAA1393; TRM5

Species reactivity:

Human

Host:

Mouse

Target:

TRMT5

Isotype:

IgG2b

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-303 of human TRMT5(NP_065861) produced in E.coli.

Clone:

LBI1B5

Conjugation:

Unconjugated

Gene name:

Homo sapiens tRNA methyltransferase 5 (TRMT5), transcript variant 3, mRNA.

Symbol:

TRMT5

Pathway:

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Function:

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Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:1000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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