Product overview:

Carrier-free (BSA/glycerol-free) AR mouse monoclonal antibody, clone LBI2E11

Field of research:

Oocyte meiosis, Pathways in cancer, Prostate cancer

Summary:

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Alternative names:

AIS; AR8; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

AR

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 420-659 of human AR(NP_000035) produced in E.coli.

Clone:

LBI2E11

Conjugation:

Unconjugated

Gene name:

Homo sapiens androgen receptor (AR), transcript variant 1, mRNA.

Symbol:

AR

Pathway:

Oocyte meiosis, Pathways in cancer, Prostate cancer

Function:

Druggable Genome, Nuclear Hormone Receptor, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:1000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com