Product overview:

Carrier-free (BSA/glycerol-free) NCF4 mouse monoclonal antibody,clone LBI12H10

Field of research:

Leukocyte transendothelial migration

Summary:

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Alternative names:

NCF; P40PHOX; SH3PXD4

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

NCF4

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human NCF4 (NP_000622) produced in HEK293T cell.

Clone:

LBI12H10

Conjugation:

Unconjugated

Gene name:

Homo sapiens neutrophil cytosolic factor 4 (NCF4), transcript variant 1, mRNA.

Symbol:

NCF4

Pathway:

Leukocyte transendothelial migration

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

IHC 1:500

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com