Product overview:

Carrier-free (BSA/glycerol-free) MGMT mouse monoclonal antibody, clone LBI10H9

Field of research:

Request info at info@sobekbio.com

Summary:

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. [UniProtKB/Swiss-Prot Function]

Alternative names:

methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741

Species reactivity:

Human

Host:

Mouse

Target:

MGMT

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human MGMT (NP_002403) produced in HEK293T cell.

Clone:

LBI10H9

Conjugation:

Unconjugated

Gene name:

Homo sapiens O-6-methylguanine-DNA methyltransferase (MGMT), mRNA.

Symbol:

MGMT

Pathway:

Request info at info@sobekbio.com

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com