Product overview:

Carrier-free (BSA/glycerol-free) TDG mouse monoclonal antibody, clone LBI3A12

Field of research:

Base excision repair

Summary:

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]

Alternative names:

hTDG

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

TDG

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 160-253 of human TDG(NP_003202) produced in E.coli.

Clone:

LBI3A12

Conjugation:

Unconjugated

Gene name:

Homo sapiens thymine DNA glycosylase (TDG), transcript variant 1, mRNA.

Symbol:

TDG

Pathway:

Base excision repair

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com