Product overview:

Carrier-free (BSA/glycerol-free) ALAS1 mouse monoclonal antibody, clone LBI5D5

Field of research:

Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism

Summary:

This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]

Alternative names:

ALAS; ALAS-H; ALAS3; ALASH; MIG4

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

ALAS1

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 57-308 of human ALAS1(NP_000679) produced in E.coli.

Clone:

LBI5D5

Conjugation:

Unconjugated

Gene name:

Homo sapiens 5'-aminolevulinate synthase 1 (ALAS1), transcript variant 1, mRNA; nuclear gene for mitochondrial product.

Symbol:

ALAS1

Pathway:

Glycine, serine and threonine metabolism, Metabolic pathways, Porphyrin and chlorophyll metabolism

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com