Product overview:

Carrier-free (BSA/glycerol-free) RFC3 mouse monoclonal antibody,clone LBI1C7

Field of research:

DNA replication, Mismatch repair, Nucleotide excision repair

Summary:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Alternative names:

RFC38

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

RFC3

Isotype:

IgG2a

Immunogen:

Full length human recombinant protein of human RFC3 (NP_002906) produced in E.coli.

Clone:

LBI1C7

Conjugation:

Unconjugated

Gene name:

Homo sapiens replication factor C subunit 3 (RFC3), transcript variant 1, mRNA.

Symbol:

RFC3

Pathway:

DNA replication, Mismatch repair, Nucleotide excision repair

Function:

Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000, IHC 1:500

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com