Product overview:

Carrier-free (BSA/glycerol-free) TYMS mouse monoclonal antibody, clone LBI9H4

Field of research:

Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism

Summary:

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. [provided by RefSeq, Jul 2008]

Alternative names:

HST422; TMS; TS

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

TYMS

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human TYMS (NP_001062) produced in SF9 cell.

Clone:

LBI9H4

Conjugation:

Unconjugated

Gene name:

Homo sapiens thymidylate synthetase (TYMS), transcript variant 1, mRNA.

Symbol:

TYMS

Pathway:

Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com