Product overview:

Carrier-free (BSA/glycerol-free) PCNA mouse monoclonal antibody, clone LBI3F3

Field of research:

Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

Summary:

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Alternative names:

ATLD2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

PCNA

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human PCNA (NP_002583) produced in E.coli.

Clone:

LBI3F3

Conjugation:

Unconjugated

Gene name:

Homo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1, mRNA.

Symbol:

PCNA

Pathway:

Base excision repair, Cell cycle, DNA replication, Mismatch repair, Nucleotide excision repair

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com