Product overview:

Carrier-free (BSA/glycerol-free) PHKG2 mouse monoclonal antibody, clone LBI1F9

Field of research:

Calcium signaling pathway, Insulin signaling pathway

Summary:

Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9C, also known as autosomal liver glycogenosis. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Alternative names:

GSD9C

Species reactivity:

Human, Mouse

Host:

Mouse

Target:

PHKG2

Isotype:

IgG2b

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-216 of human PHKG2 (NP_000285) produced in E.coli.

Clone:

LBI1F9

Conjugation:

Unconjugated

Gene name:

Homo sapiens phosphorylase kinase catalytic subunit gamma 2 (PHKG2), transcript variant 1, mRNA.

Symbol:

PHKG2

Pathway:

Calcium signaling pathway, Insulin signaling pathway

Function:

Druggable Genome, Protein Kinase

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:1000, IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com