Product overview:

Carrier-free (BSA/glycerol-free) APEX1 mouse monoclonal antibody, clone LBI4D9

Field of research:

Base excision repair

Summary:

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]

Alternative names:

APE; APE1; APEN; APEX; APX; HAP1; REF1

Species reactivity:

Human, Monkey, Mouse, Rat, Dog

Host:

Mouse

Target:

APEX1

Isotype:

IgG2b

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-242 of human APEX1 (NP_001632) produced in E.coli.

Clone:

LBI4D9

Conjugation:

Unconjugated

Gene name:

Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), transcript variant 1, mRNA.

Symbol:

APEX1

Pathway:

Base excision repair

Function:

Druggable Genome, Stem cell - Pluripotency, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com