Product overview:

Carrier-free (BSA/glycerol-free) PYCARD mouse monoclonal antibody, clone LBI2A7

Field of research:

Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

Summary:

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternative names:

ASC; CARD5; TMS; TMS-1; TMS1

Species reactivity:

Human

Host:

Mouse

Target:

PYCARD

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.

Clone:

LBI2A7

Conjugation:

Unconjugated

Gene name:

Homo sapiens PYD and CARD domain containing (PYCARD), transcript variant 1, mRNA.

Symbol:

PYCARD

Pathway:

Cytosolic DNA-sensing pathway, NOD-like receptor signaling pathway

Function:

Druggable Genome

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:500~2000, IHC 1:150, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com