Product overview:

Carrier-free (BSA/glycerol-free) RFC4 mouse monoclonal antibody, clone LBI1A8

Field of research:

DNA replication, Mismatch repair, Nucleotide excision repair

Summary:

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Alternative names:

A1; RFC37

Species reactivity:

Human, Monkey, Mouse, Rat, Dog

Host:

Mouse

Target:

RFC4

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human RFC4(NP_002907) produced in HEK293T cell.

Clone:

LBI1A8

Conjugation:

Unconjugated

Gene name:

Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.

Symbol:

RFC4

Pathway:

DNA replication, Mismatch repair, Nucleotide excision repair

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:200~4000, IHC 1:150, IF 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com