Product overview:

Carrier-free (BSA/glycerol-free) SAE1 mouse monoclonal antibody, clone LBI1G8

Field of research:

Ubiquitin mediated proteolysis

Summary:

Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]). [supplied by OMIM, Mar 2010]

Alternative names:

AOS1; HSPC140; SUA1; UBLE1A

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

SAE1

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human SAE1(NP_005491) produced in HEK293T cell.

Clone:

LBI1G8

Conjugation:

Unconjugated

Gene name:

Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1, mRNA.

Symbol:

SAE1

Pathway:

Ubiquitin mediated proteolysis

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:4000, IHC 1:150, IF 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com