Product overview:

Carrier-free (BSA/glycerol-free) RFC2 mouse monoclonal antibody, clone LBI4E1

Field of research:

DNA replication, Mismatch repair, Nucleotide excision repair

Summary:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Alternative names:

RFC40

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

RFC2

Isotype:

IgG1

Immunogen:

Human recombinant protei fragment corresponding to amino acids 1-234 of human RFC2(NP_002905) produced in E.coli.

Clone:

LBI4E1

Conjugation:

Unconjugated

Gene name:

Homo sapiens replication factor C subunit 2 (RFC2), transcript variant 2, mRNA.

Symbol:

RFC2

Pathway:

DNA replication, Mismatch repair, Nucleotide excision repair

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:200~2000, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com