Product overview:

Carrier-free (BSA/glycerol-free) UBE2E3 mouse monoclonal antibody, clone LBI1D10

Field of research:

Ubiquitin mediated proteolysis

Summary:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternative names:

UBCH9; UbcM2

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

UBE2E3

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of human UBE2E3(NP_006348) produced in E.coli.

Clone:

LBI1D10

Conjugation:

Unconjugated

Gene name:

Homo sapiens ubiquitin conjugating enzyme E2 E3 (UBE2E3), transcript variant 1, mRNA.

Symbol:

UBE2E3

Pathway:

Ubiquitin mediated proteolysis

Function:

Request info at info@sobekbio.com

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

Form:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Applications & dilution:

WB 1:2000, FLOW 1:100

Storage buffer:

Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)

Storage:

Stable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. Aliquot will be stable at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com