Product info:

This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.

Alternative names:

ATG7; APG7-LIKE; APG7L; GSA7; Apg7/Atg7

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Preautophagosomal structure

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 500-676 of human ATG7 (NP_001129503.2).

Positive control:

Jurkat, Rat testis

AA Sequence:

LGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI

Purification:

Affinity purification

Molecular weight:

Calculated MW: 68kDa/75kDa/77kDa Observed MW: 78KDa

Form:

Liquid

Applications:

IF (Human colon cancer cell, U-87MG cells, Mouse adenocarcinoma, Mus musculus, Sus scrofa) WB (Human chronic myeloid leukemia cells, U-87MG cells, Mouse cells, HeLa, Danio rerio, Mus musculus, Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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