Product info:

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Alternative names:

ADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT; m.HsaI; DNMT1

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Nucleus

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).

Positive control:

293T, SW480

AA Sequence:

MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 144kDa/183kDa/184kDa Observed MW: 200kDa

Form:

Liquid

Applications:

WB (Human hepatocellular carcinoma cell line, HUVEC, SMMC7721, BEL7404, Human cells, HEK293T, BEL-7402, Homo sapiens, Mus musculus, Rattus norvegicus) IF (Leporidae, Homo sapiens, Rattus norvegicus) IHC (Rattus norvegicus) ChIP (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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