Product info:

This gene encodes a member of the glycosyltransferase O-Fuc family. This enzyme adds O-fucose through an O-glycosidic linkage to conserved serine or threonine residues in the epidermal growth factor-like repeats of a number of cell surface and secreted proteins. O-fucose glycans are involved in ligand-induced receptor signaling. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternative names:

POFUT1; DDD2; FUT12; O-FUT; O-Fuc-T; O-FucT-1; OFUCT1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Endoplasmic reticulum

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 28-194 of human POFUT1 (NP_758436.1).

Positive control:

HepG2, HL-60, Mouse heart, Mouse liver

AA Sequence:

SWDPAGYLLYCPCMGRFGNQADHFLGSLAFAKLLNRTLAVPPWIEYQHHKPPFTNLHVSYQKYFKLEPLQAYHRVISLEDFMEKLAPTHWPPEKRVAYCFEVAAQRSPDKKTCPMKEGNPFGPFWDQFHVSFNKSELFTGISFSASYREQWSQRRENHSCVTLLFPR

Purification:

Affinity purification

Molecular weight:

Calculated MW: 22kDa/43kDa Observed MW: 44kDa

Form:

Liquid

Applications:

WB (HepG2, Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:100 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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