Product info:

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Alternative names:

PCK1; PEPCK-C; PEPCK1; PEPCKC

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 520 to the C-terminus of human PCK1 (NP_002582.3).

Positive control:

HepG2, Mouse liver, Mouse kidney, Rat liver, Rat pancreas, Rat kidney

AA Sequence:

DKEGKFLWPGFGENSRVLEWMFNRIDGKASTKLTPIGYIPKEDALNLKGLGHINMMELFSISKEFWEKEVEDIEKYLEDQVNADLPCEIEREILALKQRISQM

Purification:

Affinity purification

Molecular weight:

Calculated MW: 34kDa/69kDa Observed MW: 69KDa

Form:

Liquid

Applications:

WB (Primary Mouse liver, HEK293T, MCF7, Mus musculus)

Dilution:

WB 1:1000 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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Email: info@sobekbio.com

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