Product info:

Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants.

Alternative names:

DCP1A; HSA275986; Nbla00360; SMAD4IP1; SMIF

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus, P-body

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human DCP1A (NP_060873.4).

Positive control:

HepG2, Jurkat, Mouse brain, Rat brain

AA Sequence:

NEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPRQRSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLTPQHDQIQTQPLGKGAMVASFSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEVFVQPKVLSSAIPVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 59kDa/63kDa Observed MW: 70KDa

Form:

Liquid

Applications:

IF (HeLa, RD cell) WB (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IF 1:20 - 1:50

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com