Product info:

Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1, thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6-methyladenosine DNA, thereby regulating Polycomb silencing (By similarity.

Alternative names:

ALKBH4; ABH4

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Cytoplasm, Midbody, Nucleus

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ALKBH4 (NP_060091.1).

Positive control:

SKOV3, NCI-H125, MCF7

AA Sequence:

MAAAAAETPEVLRECGCKGIRTCLICERQRGSDPPWELPPAKTYRFIYCSDTGWAVGTEESDFEGWAFPFPGVMLIEDFVTREEEAELVRLMDRDPWKLSQSGRRKQDYGPKVNFRKQKLKTEGFCGLPSFSREVVRRMGLYPGLEGFRPVEQCNLDYCPERGSAIDPHL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 4kDa/33kDa Observed MW: 36kDa

Form:

Liquid

Applications:

WB (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com