Product info:

Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7G caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7G cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs. Plays a role in transcriptional regulation.

Alternative names:

TGS1; NCOA6IP; PIMT; PIPMT

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Cajal body, Cytoplasm, Nucleus

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 714-853 of human TGS1 (NP_079107.6).

Positive control:

Jurkat, MCF7, 293T, Mouse thymus, Mouse testis

AA Sequence:

RVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET

Purification:

Affinity purification

Molecular weight:

Calculated MW: 96kDa Observed MW: 97kDa

Form:

Liquid

Applications:

IF (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com