Product info:

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative names:

SPTBN1; ELF; HEL102; SPTB2; betaSpII; spectrin beta

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Cell membrane, Cytoplasm, Cytoplasmic side, M line, Peripheral membrane protein, cytoskeleton, myofibril, sarcomere

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 2100-2200 of human SPTBN1 (NP_003119.2).

Positive control:

U-87MG, HeLa, Jurkat, LO2, 293T, Mouse brain

AA Sequence:

PPSPEPSTKVSEEAESQQQWDTSKGEQVSQNGLPAEQGSPRMAETVDTSEMVNGATEQRTSSKESSPIPSPTSDRKAKTALPAQSAATLPARTQETPSAQM

Purification:

Affinity purification

Molecular weight:

Calculated MW: 251kDa/253kDa/274kDa Observed MW: 275kDa

Form:

Liquid

Applications:

IF (Homo sapiens)

Dilution:

WB 1:1000 - 1:3000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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