Product info:

Deubiquitinating enzyme involved in DNA repair and reassembly of the Golgi apparatus and the endoplasmic reticulum following mitosis. Necessary for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity. Plays a role in VCP-mediated formation of transitional endoplasmic reticulum (tER (By similarity. Mediates dissociation of the ternary complex containing STX5A, NSFL1C and VCP (By similarity. Also involved in DNA repair following phosphorylation by ATM or ATR: acts by catalyzing deubiquitination of SPRTN, thereby promoting SPRTN recruitment to chromatin and subsequent proteolytic cleavage of covalent DNA-protein cross-links (DPCs. Hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains.

Alternative names:

VCPIP1; DUBA3; VCIP135

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Endoplasmic reticulum, Golgi apparatus, Golgi stack

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 923-1222 of human VCPIP1 (NP_079330.2).

Positive control:

MCF7, HeLa

AA Sequence:

VFYSIMKKTMGMADGKHCTFPHLPGKTFVYNASEDRLELCVDAAGHFPIGPDVEDLVKEAVSQVRAEATTRSRESSPSHGLLKLGSGGVVKKKSEQLHNVTAFQGKGHSLGTASGNPHLDPRARETSVVRKHNTGTDFSNSSTKTEPSVFTASSSNSELIRIAPGVVTMRDGRQLDPDLVEAQRKKLQEMVSSIQASMDRHLRDQSTEQSPSDLPQRKTEVVSSSAKSGSLQTGLPESFPLTGGTENLNTETTDGCVADALGAAFATRSKAQRGNSVEELEEMDSQDAEMTNTTEPMDHS

Purification:

Affinity purification

Molecular weight:

Calculated MW: 134kDa Observed MW: 134kDa

Form:

Liquid

Applications:

WB (Mus musculus) Co-IP (Mus musculus)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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