Product info:

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternative names:

HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; PRMT5

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Golgi apparatus, Nucleus

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 40-140 of human PRMT5 (NP_006100.2).

Positive control:

Jurkat, HepG2, HeLa, NIH/3T3, PC-12, COS-1

AA Sequence:

FLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTN

Purification:

Affinity purification

Molecular weight:

Calculated MW: 53kDa/66kDa/67kDa/71kDa/72kDa Observed MW: 70kDa

Form:

Liquid

Applications:

WB (Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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