Product info:

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Alternative names:

BUB1B; BUB1beta; BUBR1; Bub1A; MAD3L; MVA1; SSK1; hBUBR1

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human BUB1B (NP_001202.4).

Positive control:

293T, U-87MG, HeLa, K-562, Mouse thymus, Mouse testis

AA Sequence:

EEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEKKEKMMYCK

Purification:

Affinity purification

Molecular weight:

Calculated MW: 105kDa/119kDa/121kDa Observed MW: 130kDa

Form:

Liquid

Applications:

WB (Homo sapiens)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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