Product info:

This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

Alternative names:

ARHGEF7; BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB

Species reactivity:

Human, Mouse, Monkey

Host:

Rabbit

Cellular localisation:

Cell junction, Cell projection, Cytoplasm, cell cortex, focal adhesion, lamellipodium, ruffle

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_001106984.1).

Positive control:

SW620, Jurkat, COS-1, COS-7, MCF7, K-562, Mouse lung, Mouse heart

AA Sequence:

MNSAEQTVTWLITLGVLESPKKTISDPEGFLQASLKDGVVLCRLLERLLPGTIEKTFDANDLYQGQNFNKVLSSLVTLNKVTADIGLGSDSVCARPSSHRIKSFDSLGSQSLHTRTSKLFQGQYRSLDMTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSA

Purification:

Affinity purification

Molecular weight:

Calculated MW: 70kDa/73kDa/82kDa/84kDa/87kDa/90kDa Observed MW: 86kDa

Form:

Liquid

Applications:

IHC (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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