Product info:

This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants.

Alternative names:

RAP1A; C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell junction, Cell membrane, Cytoplasm, Early endosome, Lipid-anchor, perinuclear region

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Rap1A (NP_002875.1).

Positive control:

U-87MG, HL-60, Mouse lung, Mouse kidney, Rat kidney, Rat brain

AA Sequence:

QCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKS

Purification:

Affinity purification

Molecular weight:

Calculated MW: 20kDa Observed MW: 21kDa

Form:

Liquid

Applications:

WB (Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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