Product info:

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis.

Alternative names:

NODAL; HTX5

Species reactivity:

Human, Rat

Host:

Rabbit

Cellular localisation:

Secreted

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 198-347 of human NODAL (NP_060525.3).

Positive control:

SH-SY5Y, HepG2, 293T, Rat liver

AA Sequence:

SNLSQEQRQLGGSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 39kDa Observed MW: 44kDa

Form:

Liquid

Applications:

IHC (Human glioma tissue)

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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