Product info:

Lipid transfer protein involved in autophagosome assembly. Tethers the edge of the isolation membrane (IM to the endoplasmic reticulum (ER and mediates direct lipid transfer from ER to IM for IM expansion. Binds to the ER exit site (ERES, which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B to the IM for membrane expansion. Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P-containing membranes. Also regulates lipid droplets morphology and distribution within the cell.

Alternative names:

ATG2A

Species reactivity:

Human

Host:

Rabbit

Cellular localisation:

Lipid droplet, Peripheral membrane protein, Preautophagosomal structure membrane

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human ATG2A (NP_055919.2).

Positive control:

U-87MG, HeLa

AA Sequence:

DLHGIYEDGGKPPVPCLRVSKALDPKSTGRKYFLPQVVVTVNPQSSSTQWEVAPEKGEELELSVESPCELREPEPSPFSSKRTMYETEEMVIPGDPEEMRT

Purification:

Affinity purification

Molecular weight:

Calculated MW: 13kDa/35kDa/212kDa/213kDa Observed MW: 213kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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