Product info:

Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C at position 34 of mt-tRNA(Met. mt-tRNA(Met methylation at cytosine(34 takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5c at this position. mt-tRNA(Met containing the f(5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.

Alternative names:

NSUN3; MST077; MSTP077

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

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Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human NSUN3 (NP_071355.1).

Positive control:

K-562, Mouse thymus, Rat thymus

AA Sequence:

MLTQLKAKSEGKLAKQICKVVLDHFEKQYSKELGDAWNTVREILTSPSCWQYAVLLNRFNYPFELEKDLHLKGYHTLSQGSLPNYPKSVKCYLSRTPGRIPSERHQIGNLKKYYLLNAAS

Purification:

Affinity purification

Molecular weight:

Calculated MW: 38kDa Observed MW: 38kDa

Form:

Liquid

Applications:

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Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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