Product info:

Scaffolding protein that enhances integrin activation mediated by TLN1 and/or TLN2, but activates integrins only weakly by itself. Binds to membranes enriched in phosphoinositides. Enhances integrin-mediated cell adhesion onto the extracellular matrix and cell spreading; this requires both its ability to interact with integrins and with phospholipid membranes. Required for the assembly of focal adhesions. Participates in the connection between extracellular matrix adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits FBLIM1 to focal adhesions. Plays a role in the TGFB1 and integrin signaling pathways. Stabilizes active CTNNB1 and plays a role in the regulation of transcription mediated by CTNNB1 and TCF7L2/TCF4 and in Wnt signaling.

Alternative names:

FERMT2; KIND2; MIG2; PLEKHC1; UNC112; UNC112B; mig-2

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell junction, Cell projection, Cell surface, Cytoplasm, Cytoplasmic side, I band, Membrane, Nucleus, Peripheral membrane protein, cell cortex, cytoskeleton, focal adhesion, lamellipodium membrane, myofibril, sarcomere

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 380-550 of human FERMT2 (NP_006823.1).

Positive control:

A-549, HT-29, LO2, MCF7, HT-1080, Mouse liver

AA Sequence:

KVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSFLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMS

Purification:

Affinity purification

Molecular weight:

Calculated MW: 72kDa/77kDa/78kDa Observed MW: 78kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:1000 - 1:2000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com