Product info:

Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres cell lines and mediates sumoylation of shelterin complex (telosome components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs. Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome.

Alternative names:

SMC5; SMC5L1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Chromosome, Nucleus, PML body, telomere

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 872-1101 of human SMC5 (NP_055925.2).

Positive control:

LO2, HeLa, Jurkat, Mouse testis, Rat testis

AA Sequence:

LTEERSRASCFTGLNPTIVQEYTKREEEIEQLTEELKGKKVELDQYRENISQVKERWLNPLKELVEKINEKFSNFFSSMQCAGEVDLHTENEEDYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPINERRVFEMVVNTACKENTSQYFFITPKLLQNLPYSEKMTVLFVYNGPHMLEPNTWNLKAFQRRRRRITFTQPS

Purification:

Affinity purification

Molecular weight:

Calculated MW: 128kDa Observed MW: 132kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:1000 - 1:3000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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