Product info:

Ubiquitin-like modifier involved in intra-Golgi traffic (By similarity. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation (By similarity. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1 (By similarity. Involved in autophagy. Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.

Alternative names:

GABARAPL2; ATG8; ATG8C; GATE-16; GATE16; GEF-2; GEF2

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Cytoplasmic vesicle, Golgi apparatus, autophagosome

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1).

Positive control:

293T, Raji, Mouse brain, Mouse thymus

AA Sequence:

MKWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFGF

Purification:

Affinity purification

Molecular weight:

Calculated MW: 13kDa Observed MW: 17-18kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com