Product info:

DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes.

Alternative names:

DAD1; OST2

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Endoplasmic reticulum membrane, Multi-pass membrane protein

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAD1 (NP_001335.1).

Positive control:

A-431, Mouse thymus, Mouse brain, Mouse kidney, Mouse liver, Rat testis

AA Sequence:

MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFA

Purification:

Affinity purification

Molecular weight:

Calculated MW: 12kDa Observed MW: 22kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com