Product info:

Acts as a sulfur carrier required for 2-thiolation of mcm(5S(2U at tRNA wobble positions of cytosolic tRNA(Lys, tRNA(Glu and tRNA(Gln. Serves as sulfur donor in tRNA 2-thiolation reaction by being thiocarboxylated (-COSH at its C-terminus by MOCS3. The sulfur is then transferred to tRNA to form 2-thiolation of mcm(5S(2U. Also acts as a ubiquitin-like protein (UBL that is covalently conjugated via an isopeptide bond to lysine residues of target proteins such as MOCS3, ATPBD3, CTU2, USP15 and CAS. The thiocarboxylated form serves as substrate for conjugation and oxidative stress specifically induces the formation of UBL-protein conjugates.

Alternative names:

URM1; C9orf74

Species reactivity:

Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-63 of human URM1 (NP_001252511.1).

Positive control:

Rat eye

AA Sequence:

MAAPLSVEVEFGGGAELLFDGIKKHRVTLPGQEEPWDIRNLLIWIKKNLLKERPELFIQGDSV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 7kDa/11kDa/15kDa Observed MW: 11kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com