Product info:

As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton complex involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Probable anchoring protein which tethers the nucleus to the cytoskeleton by binding PLEC which can associate with the intermediate filament system. Plays a role in the regulation of aortic epithelial cell morphology, and is required for flow-induced centrosome polarization and directional migration in aortic endothelial cells.

Alternative names:

SYNE3; C14orf139; C14orf49; KASH3; LINC00341; NCRNA00341; NET53; Nesp3; nesprin-3

Species reactivity:

Human, Mouse,

Host:

Rabbit

Cellular localisation:

Nucleus envelope, Nucleus outer membrane, Rough endoplasmic reticulum, Single-pass type IV membrane protein

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 740-920 of human SYNE3 (NP_689805.3).

Positive control:

Jurkat, K-562, LO2, HepG2

AA Sequence:

LRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLF

Purification:

Affinity purification

Molecular weight:

Calculated MW: 68kDa/111kDa/112kDa Observed MW: 112kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com