Product info:

Catalytic subunit of tRNA (adenine-N(1--methyltransferase, which catalyzes the formation of N(1-methyladenine at position 58 (m1A58 in initiator methionyl-tRNA. Catalytic subunit of mRNA N(1-methyltransferase complex, which mediates methylation of adenosine residues at the N(1 position of a small subset of mRNAs: N(1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.

Alternative names:

TRMT61A; C14orf172; GCD14; Gcd14p; TRM61; hTRM61

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Nucleus

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).

Positive control:

PC-3, U-251MG, A-549, Mouse spleen, Rat testis

AA Sequence:

MSFVAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRGGWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIRTIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHVADAVFLDIPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVRTVSLPPPDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFATKTPG

Purification:

Affinity purification

Molecular weight:

Calculated MW: 31kDa Observed MW: 33KDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:1000 - 1:2000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com