Product info:

Guanine nucleotide exchange factor (GEF, which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i-alpha protein, possibly leading to release G(i-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i-alpha-GDP complex (By similarity. Also acts as an activator for G(q-alpha (GNAQ protein by enhancing the G(q-coupled receptor-mediated ERK activation.

Alternative names:

RIC8A; RIC8

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell membrane, Cytoplasm

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 362-531 of human RIC8A (NP_001273063.1).

Positive control:

Raji, HeLa, HepG2, 293T, DU145, Mouse brain, Rat brain

AA Sequence:

DVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD

Purification:

Affinity purification

Molecular weight:

Calculated MW: 58kDa/59kDa/60kDa Observed MW: 60kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com