Product info:

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

Alternative names:

ATP2B; DAR; DD; SERCA2; ATP2A2/SERCA2; ATP2A2

Species reactivity:

Mouse, Rat

Host:

Rabbit

Cellular localisation:

Endoplasmic reticulum membrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human SERCA2/ATP2A2 (NP_733765.1).

Positive control:

Mouse heart

AA Sequence:

NAENAIEALKEYEPEMGKVYRQDRKSVQRIKAKDIVPGDIVEIAVGDKVPADIRLTSIKSTTLRVDQSILTGESVSVIKHTDPVPDPRAVNQDKKNMLFSGTNIAAGKAMGVVVATGVNTEIGKIRDEMVATEQERTPLQQKL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 109kDa/111kDa/114kDa Observed MW: 114kDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com