Product info:

Mitochondrial membrane ATP synthase (F(1F(0 ATP synthase or Complex V produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1 - containing the extramembraneous catalytic core and F(0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Key component of the proton channel; it may play a direct role in the translocation of protons across the membrane.

Alternative names:

MT-ATP6; ATPase6; MTATP6; ATP6

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Mitochondrion inner membrane, Multi-pass membrane protein

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of mouse ATP6 (NP_904333.1).

Positive control:

NCI-H460, SKOV3, MCF7, HeLa, Mouse brain, Mouse skeletal muscle

AA Sequence:

MNLSMAIPLWAGAVITGFRHKLKSSLAHFLPQGTPISLIPMLIIIETISLFIQPMALAVRLTANITAGHLLMHLIGGATLVLMNISPPTATITFIILLLLTILEFAVALIQAYVFTLLVSLYLHDNT

Purification:

Affinity purification

Molecular weight:

Calculated MW: 25kDa Observed MW: 20kDa

Form:

Liquid

Applications:

WB (Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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