Product info:

Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyzes the conversion of cytidine triphosphate (CTP to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV, hepatitis C virus (HCV, west Nile virus (WNV, dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV, zika virus, and human immunodeficiency virus (HIV-1. Promotes also TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR-mediated GATA3 activation and optimal T-helper 2 (Th2 cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins.

Alternative names:

RSAD2; 2510004L01Rik; cig33; cig5; vig1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasmic side, Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus, Lipid droplet, Mitochondrion, Mitochondrion inner membrane, Mitochondrion outer membrane, Peripheral membrane protein

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 42-361 of human RSAD2 (NP_542388.2).

Positive control:

PC-3, Mouse spleen, Rat lung, Rat spleen, Rat kidney

AA Sequence:

LATKRRKQQLVLRGPDETKEEEEDPPLPTTPTSVNYHFTRQCNYKCGFCFHTAKTSFVLPLEEAKRGLLLLKEAGMEKINFSGGEPFLQDRGEYLGKLVRFCKVELRLPSVSIVSNGSLIRERWFQNYGEYLDILAISCDSFDEEVNVLIGRGQGKKNHVENLQKLRRWCRDYRVAFKINSVINRFNVEEDMTEQIKALNPVRWKVFQCLLIEGENCGEDALREAERFVIGDEEFERFLERHKEVSCLVPESNQKMKDSYLILDEYMRFLNCRKGRKDPSKSILDVGVEEAIKFSGFDEKMFLKRGGKYIWSKADLKLDW

Purification:

Affinity purification

Molecular weight:

Calculated MW: 42kDa Observed MW: 46KDa

Form:

Liquid

Applications:

Request info at info@sobekbio.com

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com