Product info:

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Alternative names:

4E-BP1; 4EBP1; BP-1; PHAS-I; EIF4EBP1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

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Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human eIF4EBP1 (NP_004086.1).

Positive control:

MCF7, HeLa, SH-SY5Y, NIH/3T3

AA Sequence:

TRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI

Purification:

Affinity purification

Molecular weight:

Calculated MW: 12kDa Observed MW: 17-19kDa

Form:

Liquid

Applications:

WB (Mus musculus, Homo sapiens, Macaca mulatta)

Dilution:

WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:100 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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