Product info:

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.

Alternative names:

SNAP25; CMS18; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP; bA416N4.2; dJ1068F16.2

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell junction, Cell membrane, Cytoplasm, Lipid-anchor, perinuclear region, synapse, synaptosome

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-206 of human SNAP25 (NP_003072.2).

Positive control:

SH-SY5Y, Mouse brain, Rat brain

AA Sequence:

MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

Purification:

Affinity purification

Molecular weight:

Calculated MW: 23kDa Observed MW: 25KDa

Form:

Liquid

Applications:

WB (mouse brain, Mus musculus, Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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