Product info:

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site.

Alternative names:

VEGFA; MVCD1; VEGF; VPF; L VEGFA; VEGF A

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Secreted

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 111-161 of human VEGF (NP_001165094).

Positive control:

293T, HeLa, Mouse lung

AA Sequence:

PHQGQHIGEMSFLQHNKCECRPKKDRARQEKKSVRGKGKGQKRKRKKSRYK

Purification:

Affinity purification

Molecular weight:

Calculated MW: 15-27kDa/34-45kDa Observed MW: 15KDa

Form:

Liquid

Applications:

WB (Oryctolagus cuniculus, Homo sapiens, Mus musculus, Rattus norvegicus) IF (Homo sapiens) IHC (Rattus norvegicus, Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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