Product info:

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.

Alternative names:

His; His tag; His-tag

Species reactivity:

Request info at info@sobekbio.com

Host:

Rabbit

Cellular localisation:

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Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to His tag.

Positive control:

293T

AA Sequence:

HHHHHH

Purification:

Affinity purification

Molecular weight:

Observed MW: 35KDa/27KDa

Form:

Liquid

Applications:

IF (Homo sapiens, Danio rerio) WB (Danio rerio, Homo sapiens)

Dilution:

WB 1:1000 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com